Human 1 3 galactosyltransferase enzyme were 4IgB7H3 7 on the modification of.docVIP

 PAGE \* MERGEFORMAT 17 Human 1 3 galactosyltransferase enzyme were 4IgB7H3 7 on the modification of Of: Tang Chunhua, Hot Separation, Yue love ring, running Li, Jiang Junhua Zhang Hao Cao Jingyuan, Zhou Ying Council, WU Shi-liang [Abstract] Objective: To investigate 4IgB7 H3 gene transfection of human gastric cancer cell line SGC7901 after the human 1, 3 galactosyltransferase enzyme 7 (1, 3 galactosyltransferase 7, 3GalT7 expression changes in order to find 4IgB7 H3 and 3GalT7 each other. Methods: Liposome recombinant retroviral vector pEGZ Term/4IgB7 H3 transfected SGC7901 cells stably expressing 4IgB7 H3 screened the subcloned cell lines by RT PCR and Western blot used to detect the expression of 3GalT7. Results: successfully 4IgB7 H3 subclone stably expressing cell lines SGC7901/4IgB7 H3, 3GalT7 4IgB7 H3 expression with increases in the regulated. Conclusion: 4IgB7 H3 protein modification may be required 3GalT7 mature role. [Keywords:] 4IgB7 H3, 1, 3 galactosyltransferase enzyme 7, SGC7901, transfection [Abstract] Objective: We researched the change of UDP Gal: betaGlcNAc beta 1,3 galactosyltransferase polypeptide 7 expression in SGC7901/4IgB7 H3 cell to find the relation between 4IgB7 H3 and 3GalT7. Methods: Transfered pEGZ Term/4IgB7 H3 into SGC7901 cell, then detected the expression of UDP Gal: betaGlcNAc beta 1,3 galactosyltransferase polypeptide 7 with RT PCR and Western blotting. Results: SGC7901/4IgB7 H3 cell line were successfully obtained. The expression of 3GalT7 was up regulated follow the up regulation of 4IgB7 H3 on mRNA and protein level. Conclusion: UDP Gal: betaGlcNAc beta 1,3 galactosyltransferase polypeptide 7 may modify 4IgB7 H3 protein. [Keywords:] 4IgB7 H3, 1, 3 galactosyltransferase 7, SGC7901, transfer Costimulatory molecules (costimulatory molecules) B7 H3 was first reported in 2001 by Dong et al [1], from human dendritic cells derived from cloned cDNA library for the newly discovered B7 family members. This gene is