Human peritoneal mesothelial cells in vitro.docVIP

 PAGE \* MERGEFORMAT 13 Human peritoneal mesothelial cells in vitro [Abstract] Objective: To establish a simple and effective human peritoneal mesothelial cells (HPMC) in vitro methods. Methods: with 0.2% trypsin - 0.02% EDTANa2 digested the NPC retina tissue, cell suspension was filtered through 100 mesh after the training. Using morphology, immunohistochemistry (SP method) Identification of cells. Results: The isolation and culture of cells under light microscope showed cobblestone-like paving. Immunohistochemical identification showed keratin, vimentin antigen positive, the first Ⅷ factor, leukocyte CD45 antigen-negative, confirmed that cultured cells are indeed HPMC. Conclusion: The trypsin digestion method is a simple and practical method of separation of HPMC. [Keywords:] peritoneal mesothelial cells; trypsin; greater omentum Abdominal adhesions are a common complication after abdominal surgery, often caused by intestinal obstruction, chronic abdominal pain, and infertility, infertility, etc., but adhesion is difficult to be improved through re-operation [1]. In recent years, the formation mechanism of intra-abdominal adhesions have more in-depth study, that the intra-abdominal adhesion formation and a variety of factors, including the integrity of the peritoneal mesothelial cells and function normally or not is a key factor in intra-abdominal adhesion formation [2]. Therefore, the establishment of human peritoneal mesothelial cell culture system for in vitro study of human peritoneal mesothelial cells and its physiological function in the role of intra-abdominal adhesion prevention and treatment provided an effective means. Early in the 20th century, early 80s, Wu et al [3] by directly obtained from the patient’s ascites method of peritoneal mesothelial cells to successfully cultivate a human peritoneal mesothelial cells. After Kern [4], and Stylianou [5], respectively, by collagenase digestion method and the direct planting method, cultivate human