Huaimi solid-state fermentation to improve the study of quercetin content in.docVIP

 PAGE \* MERGEFORMAT 13 Huaimi solid-state fermentation to improve the study of quercetin content in Author: Xu Mengmeng, Shen Jing, Xu Chun, Wang Su, Wang Hong, Sun Qiling [Abstract] Objective screening the lab received a solid-state fermentation of Aspergillus niger Huaimi and carried out optimization of culture conditions, so that it will be Huaimi into quercetin in rutin and improve the content of quercetin in Sophora japonica . Method uses orthogonal experiment on seed culture conditions and fermentation conditions were optimized. Results The optimized biomass up to 8.32 g / L, optimal conditions for solid-state fermentation of rutin conversion rate can reach 98%. The use of high-performance liquid chromatography of fermentation products were identified to prove that the product is converted quercetin. Conclusion Huaimi solid-state fermentation method of micro-organisms that can improve the content of quercetin in Sophora japonica. [Keywords:] Huaimi; rutin; quercetin; Aspergillus niger; Microbial Transglutaminase Abstract: Objective Flos Sophorae Immaturus was subjected to solid state fermentation process by an Aspergillus niger strain to transform rutin into quercetin. MethodsThe conditions of culture and fermentation process were optimized by the orthogonal layout. Results By modulating the conditions, the biomass could reach 8.32 g / L and the transformation rate of rutin could reach 98%. It was proved that the product was quercetin by HPLC. ConclusionThe content of quercetin in Flos Sophorae Immaturus can be increased by the method of microbial transformation. Keywords:: Flos Sophorae Immaturus; Rutin; Quercetin; Aspergillus niger; Microbial transformation Sophora japonica (Flos Sophorae Immaturus) Alias: Baihuai, pomelo flower, for leguminous plants Sophora japonica Sophora japonica L. flower buds. With the cooling blood to stop bleeding, Liver Xiehuo function. Used for blood in the stool, hemorrhoids blood, Campylobacter, uterine bleeding, v