HPLC determination of anti-inflammatory antipyretic particle content of caffeic acid.docVIP

 PAGE \* MERGEFORMAT 14 HPLC determination of anti-inflammatory antipyretic particle content of caffeic acid Author: Fang Chien-ho, XIONG Sheng-quan, Yan Dongmei, Zhu Wei-feng 【Abstract】 Objective high-performance liquid chromatography using anti-inflammatory antipyretic particle content of caffeic acid. Methods 18 alkyl silane bonded silica gel column (Alltima, 5 μm, 4.6 mm × 250 mm); mobile phase: acetonitrile -0.4% phosphoric acid (eighty-seven past one p.m.); detection wavelength of 323 nm. The results of caffeic acid into the sample volume in the range of 0.0387 ~ 0.387 0 μg good linear relationship; r = 0.9998 (n = 6), the average recovery was 100.56% (n = 6); precision of RSD = 1.36% (n = 6). Conclusion The method is simple, rapid and accurate. Keywords: high-performance liquid chromatography; anti-inflammatory antipyretic particle; caffeic acid Abstract: ObjectiveTo establish an HPLC method for determination of Caffeic acid in Xiaoyan Tuire Granule.MethodsThe separation was carried out. ODS column (AIltima, 5 μm, 4.6 mm × 250 mm) was used mobile phase of Acetonitrile-0.4% Phosphoric acid (13 : 87) and detection wavelength of 323 nm.ResultsThe Caffeic acid samples showed a good linear relationship at the range of 0.0387 ~ 0.387 0 μg (r = 0.9998, n = 6). The average recovery of the added sample was 100.56% (n = 6) and method precision was RSD = 1.36% (n = 6). ConclusionThe method is simple, available and accurate Keywords:: HPLC; Xiaoyan Tuire Granule; Caffeic acid Prescription anti-inflammatory antipyretic particles derived from “The People’s Republic of China Ministry of Health, Drug Standards No. 5 Chinese patent medicines Narikata agents” [1] anti-inflammatory antipyretic granules. All side by Daqingye, dandelion, Chinese violet, licorice, etc., has a heat-clearing and detoxifying, cooling blood swelling for a cold fever, upper respiratory tract infection, sore throat and all kinds of sore throat, etc. boil. Pharm