HPLC detection of ivermectin carp muscle.docVIP

 PAGE \* MERGEFORMAT 12 HPLC detection of ivermectin carp muscle [Abstract] carp muscle were used to establish a simple and accurate RP-HPLC method for the drug ivermectin residues in fish detection and pharmacokinetic study. Ivica carp muscle streptozotocin extracted with ethyl acetate, the supernatant after centrifugation and evaporated to dryness at 45 ℃, the residue with methanol water (4:1, V / V) mixture dissolved in hexane fat, remove the clear after centrifugation solution, 20 μL injection for HPLC analysis. extractant investigated in this way, the impact of column temperature and mobile phase was determined to ethyl acetate as extraction solvent, water temperature was 25 ℃ and methanol (90:10, V / V) Optimal conditions for the mobile phase. The linear range is 0.025 ~ 2.50 μg / g, the correlation coefficient r = 0.9997, detection limit was 11 ng / g. The intra RSD 1.0% 5.8% 1.0% day RSD ~ 7.7%, relative recovery (99.8 + -4.1)%, the absolute recovery was (95.9 + -3.9)%. The method is simple, accurate and reliable. [Keywords:] ivermectin, crucian carp, muscle, residues, HPLC 1 Introduction Ivermectin (ivermectin, IVM) is a derivative of avermectin, is one of the best broad-spectrum antiparasitic drugs, with a broad spectrum, high efficiency, small dosage, safety, etc., for internal and external parasitic organism insects, nematodes and arthropods are particularly efficient role of .1981 killed in flooding into the market, in clinical veterinary medicine has been widely used. and its parasitic disease control in aquaculture in the application also caused concern. now , ivermectin residues in animal methods include HPLC UV detection method [1] and HPLC with fluorescence detection method [2,3]. However, these methods are mostly animal drug residues in samples of fish Determination of IVM little studied. Pantelis et al [4] using enzyme-linked immunosorbent assay (ELISA) were determined within the IVM concentration in the sea bream; Zhang et al [5