Human placenta hematopoietic stem - progenitor cells from enrichment.docVIP

 PAGE \* MERGEFORMAT 3 Human placenta hematopoietic stem / progenitor cells from enrichment Authors: Zhang Tao, Dai-Xiong Chen, Ning, Liu Zulin, Qi Ying, Liu Jinwei [Abstract] In order to explore the tissue from the placenta separation and enrichment of hematopoietic stem / progenitor cells (HSPC) of standardized processes, using mechanical method combined with collagenase digestion and preparation of a single cell suspension of human placenta, using hydroxyethyl starch (6% HES) method to isolate the mononuclear cells (MNC), and then by magnetic activated cell sorting method selected sub-CD34-, CD34 CD38-, CD34 CD38 3 cells subsets, flow cytometry cell sorting in various stages of for phenotypic analysis and calculate the degree of sorting cells in the enrichment and recovery. The results showed that: mechanical method combined with collagenase digestion of human placenta Preparation of single cell suspension of mononuclear cells (MNC) and the total number (12.30 ± 3.51) * 108, with the umbilical cord blood MNC contained in the original sample number (8.86 ± 5.38) * 108 difference was not statistically significant, while the percentage of CD34 + cells [(3.93 ± 2.31)%] was significantly higher than umbilical cord blood [(0.44 ± 0.29)%]. Placental tissue single cell suspension separated by 6% HES and CD34 + cells in MNC recovery were (45.3 ± 11.7)% and (51.1 ± 9.8)%; MNC by magnetic activated cell sorting, its purity and CD34 + cells in recoveries were (73.4 ± 14.1)% and (52.7 ± 11.7)%. Conclusion: This study was established by the amp;quot;mechanical method combined with collagenase digestion method-HES separation of MNC-MACS sorting target cellsamp;quot; of the separation and purification method can be obtained from the placental tissue high abundance, high-concentration, high activity of HSPC, in order to further study provides a more economical HSPC placenta, the effect better separation and enrichment programs. [Keywords:] CD34 antigen; of hemat