Human platelets freeze-dried pre-load trehalose Technology.docVIP

 PAGE \* MERGEFORMAT 2 Human platelets freeze-dried pre-load trehalose Technology Author: Fa-Qiang Lu, Jing-Han Liu, Xi-Lin Ouyang, Xi-Jin, ZHOU Jun, Zhuang Yuan [Abstract] for the study of human platelets freeze-dried trehalose pre-load techniques and methods to screen out the optimal load trehalose experimental conditions and further study of platelets under the conditions of 37 ℃ in temperature, the load trehalose, the average size of 4 hours after in vitro activation level and aggregation responses of changes in platelet intracellular trehalose drawing load efficiency and concentration with temperature, time, extracellular trehalose concentration curve, screening an appropriate load conditions, respectively, thrombin, ADP, collagen and Ristocetin four kinds of substances as inducers of platelet activation, platelet aggregation instrument used to detect platelet aggregation before and after loading trehalose reactivity, using flow cytometry analysis of platelets before and after loading trehalose the membrane surface glycoprotein molecules CD62p, PAC-1 expression in rate, plus reversible activator inhibitor PGE-1, adenosine suppressed degree of platelet activation. The results showed that: Trehalose-load efficiency and incubation time (2 hours), temperature (30-40 ℃ ) showed good linear relationship between the conditions at 37 ℃ after incubation for 4 hours up to 60% load efficiency, loading into the cell in trehalose concentration with extracellular trehalose concentration (amp;lt;50 mmol / L) elevated the increase; compared with the pre-load, mean platelet volume (MPV), platelet activation induced by agents of the four kinds of the greatest aggregation rate were not significantly sex difference (P