Hypoxic pulmonary hypertension in rats apoptosis of pulmonary artery.docVIP

 PAGE \* MERGEFORMAT 12 Hypoxic pulmonary hypertension in rats apoptosis of pulmonary artery Of: Liu Gui-Sheng Qian Zhang Zhiyuan Li Yuncheng Wang Guan Yu Yang Song [Abstract] Objective To study bcl 2, bax apoptosis-related genes in the hypoxic rat pulmonary artery expression of the pathogenesis of hypoxic pulmonary hypertension and prevention measures. Methods 36 Wistar rats were randomly divided into 3 groups, respectively normal control group, hypoxic pulmonary hypertension, low concentrations of carbon monoxide (CO) group, 12 in each group, using in situ apoptosis, in situ hybridization and immunohistochemistry were used to detect apoptosis of pulmonary artery. Results situ apoptosis detection indicated that the normal control group, hypoxic pulmonary hypertension group, low concentration of CO group, the apoptosis rates were 8.1%, 37.6%, 76.5%, significant differences between the three, situ hybridization showed that the normal control group bax , bcl 2 expression was weak hybridization. hypoxic pulmonary hypertension group showed a weak positive hybridization bax expression, low concentrations of CO group showed strong positive expression, but bcl 2 hybrid shows hypoxic pulmonary hypertension group showed strong positive expression group showed a low concentration of CO weakly positive, Immunohistochemistry results showed that low concentrations of CO group bcl 2, bax protein were increased in bax as a. hypoxic pulmonary hypertension group although it was also expressed, but weaker, and bcl 2 expression in a dominant position . Conclusions Low concentrations of CO can regulate bcl 2, bax gene expression of apoptosis, thereby promoting apoptosis of pulmonary tissue. [Keywords:] hypoxia, pulmonary hypertension, apoptosis Pulmonary hypertension (PH) causes complicated by a variety of heart, lung or pulmonary vascular diseases. Pulmonary vascular remodeling hypoxic pulmonary hypertension is considered to be the main pathological changes (1), pu