IL12 by adjusting the balance of Th1-Th2 cytokines in inhibiting pulmonary fibrosis.docVIP

 PAGE \* MERGEFORMAT 2 IL12 by adjusting the balance of Th1/Th2 cytokines in inhibiting pulmonary fibrosis [Abstract] Objective: To study interleukin  12 (Interleukin  12, IL  12) on bleomycin (Bleomycin, BLM)-induced pulmonary fibrosis in the therapeutic effect and its mechanism. Methods: completely random design will be female Wistar rats were randomly divided into four groups 60, namely, BLM model group (group 1), IL  12 intervention group (group 2), BSA treated control group (Group 3) and normal saline (NS) control group (group 4) . No. 1,2,3 group injected through the trachea BLM pulmonary fibrosis (PF) rats, 4 groups were given an equal volume of NS. tooling after intraperitoneal injection of 1 to 12, drug intervention, the first two group IL  12 solution, Group 3 for the bovine serum albumin (Bovine Serum Albumin, BSA) solution, after 7,14,28 days mold killed five rats in each group to observe the pathological changes, lung weight, lung tissue homogenate of hydroxyproline (Hydroxyproline, Hyp) changes in the level of pulmonary coefficient on the pathology slices alveolitis and fibrosis score and the percentage of gray-scale analysis. Determination of bronchoalveolar lavage fluid (Bronchoalveolar lavage fluid, BALF) leukocytes IL  4 (Interleukin  4, lL  4), γ  interferon (Interferon  gamma, INF  γ ) level, calculate the ratio of IL  4/INF  γ . Results: (1) and Group 4 comparison, Group 1 apparent alveolitis and fibrosis, lung weight, lung index and lung tissue in Hyp content increased, alveolar inflammation and fibrosis score increased, BALF of IL  4 increase, IL  4/INF  γ ratio increased The difference was statistically significant (P