Improvement of skeletal muscle fiber in ATP staining method.docVIP

 PAGE \* MERGEFORMAT 13 Improvement of skeletal muscle fiber in ATP staining method Of: Zhang Tao, Zhang Qian, Mr Hu order, Xue Qian, CHEN Dai Hung [Abstract] Objective The modified Guth Samaha of myosin ATP staining method to observe the rat skeletal muscle fiber in dyeing effect. Methods SD rats 8m thickness of frozen sections of biceps and chest, containing 40g / L paraformaldehyde fixative fixed 5min, Tris pre-set lotion after washing at room temperature the supernatant was incubated 15min, Tris lotion in the rinse 2 times, set in the supernatant water bath shaker at 37 for 60min incubation in the shaking , 10g / L calcium chloride, washed three times post-20g / L cobalt chloride solution 3min, washed with distilled water, at 10g / L ammonium sulfide solution and incubated for 3min, tap water 3min, conventional gradient ethanol dehydration, xylene, in the of gum were mounted, and placing under the microscope. Results chest and biceps SD rats improved Guth Samaha myosin after ATP staining can be clearly shows a bright white color of dark fiber and dark brown type Type fiber. Conclusion The modified Guth Samaha myosin ATP staining reagent preparation method is simple, streamlined operations and satisfactory muscle fiber type, etc., should draw on relevant research in the application of skeletal muscle. [Keywords:] myosin ATP enzyme, muscle fiber type, rat ABSTRACT: Objective To study the effect of self modified Guth amp; Samaha s myosin ATPase staining method on the classification of SD rat skeletal muscle fiber types. Methods 8m thick cryostat sections from rat chest muscles and biceps brachii muscles were transfected with cryostat. Myosin ATPase staining was carried out according to the following procedure: Fixing sections for 5min in fixative solution containing 40g / L paraformaldehyde; Rinsing slides in Tris rinse solution and then preincubating them in alkaline preincubation solution for 15min; Rinsing slides in Tris rinse solution tw