In the rat ovary during the estrous cycle and the expression of ovulation.docVIP

 PAGE \* MERGEFORMAT 9 In the rat ovary during the estrous cycle and the expression of ovulation Study: Long Zheng Xuezhi Xuqiu Ling Zhang Chengyu Fu Chak [Abstract] Objective: To observe the relaxin-like factor (RLF) gene in the rat ovary during the estrous cycle and ovulation, the expression of RLF in ovarian function in rats. METHODS: Using RT PCR method. Results: Adult rat ovary throughout the estrous cycle has RLFmRNA of expression, in proestrus, estrus expression in ovarian strong late diestrus and estrus ovarian weak. PMSG hCG treatment of immature rats in the lower expression of ovulation RLFmRNA. Conclusion: RLF and follicle development, ovulation, corpus luteum formation and degradation are closely related. [Keywords:] RLF, rat, estrous cycle, ovulation About the author: Zhang Chengyu, female, lecturer, master, engaged in physiology teaching and research in reproductive endocrinology, Email: relaxin-like factor (relaxin like factor, RLF is a newly discovered insulin like factor3 (INSL3 gene products, belongs to insulin - insulin-like growth factor - Relaxin (insulin-IGF-relaxin family [1]. because of RLF expressed in the gonads of gender, so the field of reproductive physiology have become another hot spot of research. At present foreign studies have focused on the RLF In male animals, and few studies of rodents, domestic RLF few experimental reports. RLFmRNA of this study was to observe the estrous cycle in rats and exogenous hormone-induced ovulation in the rat model of dynamic expression to of RLF in the role of ovarian function in rats. 1 Materials and methods 1.1 Animals and Reagents: Healthy female Wistar rats by the Second Clinical Medical College of Harbin Medical University Experimental Animal Center, natural light, water, taking any food. Pregnant mare serum (PMSG (Tianjin Laboratory Animal Center products, cashmere and promote injection sex hormone (hCG (Ningbo Hormone Products Factory products, Trizol RNA extraction kit