Inn on the HPLC method for the determination of gold granules of chlorogenic acid.docVIP

 PAGE \* MERGEFORMAT 7 Inn on the HPLC method for the determination of gold granules of chlorogenic acid Of: Li Tao, Kirin, Miao surgery, Liu Jianhua [Abstract Objective Determination of gold granules Yin chlorogenic acid. Methods Kroasil ODS column (4.6 mm × 250 mm), column temperature: room temperature; mobile phase: 0.3% acetic acid - methanol (74: 26), flow rate: 0.9 mL / min, detection wavelength: 327 nm. the results of the linear range of 65 ~ 325 μg / mL, the regression equation: A = 24 239 869C-31 292, r = 0.999 3; average recovery was 100.10%, RSD = 0.87% (n = 6). Conclusions The method can be used as granules of gold Yin method for quality control. [Key Words] high performance liquid chromatography, Kim Yan granules, chlorogenic acid, determination Abstract: Objective To establish a HPLC method to determind the content of chlorogenic acid in Jinyin Granules. Method The mobile phase was 0.3% HAc-Methonal (74:26). The UV detection wavelength was 327 nm. The mobile speed was 0.9 mL / min. Separation column was Kroasil ODS (4.6 mm × 250 mm). Result A good linearity was obtained in the range of 65 ~ 325 μg, regression A = 24 239 869C-31 292, r = 0.999 3. The average recovery was 100.10 %, RSD was 0.87% (n = 6). Conclusion The method was good for determining chlorogenic acid in Jinyin Granules. Keywords:: HPLC, Jinyin Granules, chlorogenic acid, content determination Jin Yan granules by money grass, capillaris, citrus aurantium, bear bile powder 6 taste traditional Chinese medicine extracted by water, vacuum dried and mixed with a certain amount of powdered sugar, prepared from by modern technology, with clearing and detoxifying, Lee biliary jaundice, Qi analgesic efficacy for the treatment of cholecystitis, gallstones and other diseases. capillaries of chlorogenic acid is the active ingredient in the granules, therefore, we have established the preparation of chlorogenic acid in HPLC determination, the method is rapid, accurate, high sens