Innovation ink stained cryptococcal production method - Optimization staining.docVIP

 PAGE \* MERGEFORMAT 5 Innovation ink stained cryptococcal production method - Optimization staining [Abstract] Objective To observe the stained specimen of C. neoformans optimized ink staining. Methods traditional method bacilli were mounted with the ink mixture, optimization method to broth enrichment by animals, culture and then mixed with the ink, were mounted. Two Act 100 of the producer, the results compared. The results were excellent test piece 90, 10 good films, the control group of 10 excellent films, good films 20, and poor film 70. conventional staining microscope can see, hidden coccus capsular structure fuzzy, uneven distribution of bacteria, the concentration of too light or too thick ink, and his observation, optimal staining microscope specimens stained the cryptococcal capsule structure is clear, bacterial distribution, more than blastospores structure. Conclusion optimization method produced samples are retained for a long time, especially for simple operation and a large number of producers and teaching laboratories, etc.. [Keywords:] neoformans, Cryptococcus ink staining, specimen pieces India ink staining, cryptococcal pressed to reflect the morphological characteristics of C. neoformans, easy microscopic examination, the clinical identification of C. neoformans is very important and important teaching specimens. But some specimens can not be kept longer, capsular structures were observed fuzzy, less structure, blastospores, and the structure is not clear enough. After years of trial and error we have in the traditional method based on the number of improvements were made, are reported below. 1 Materials and methods 1.1 bacteria C. neoformans. 1.2 Preparation of Sabouraud medium Peptone 10g, glucose 40g, agar 1g, distilled water 1 000mL, 68.95kpa, 20min, standby. 1.3 Preparation of dye ink glycerin Ink 50mL, glycerin, phenol the 20mL, mix shake aside. Links to free paper download 1.4 Methods 1.4.1 set up