Ischemic stroke patients with blood stasis syndrome of MTHFR gene polymorphism_0.docVIP

 PAGE \* MERGEFORMAT 12 Ischemic stroke patients with blood stasis syndrome of MTHFR gene polymorphism OF: Juan lumber, Wang Zhong, Li Tao, Chang Hsiao-yen, Jing Zhiwei, Zhou Xiu, Su Liya [Abstract] Objective To investigate the N5, N10 methylenetetrahydrofolate reductase (MTHFR) gene C677T polymorphism and ischemic stroke with blood stasis syndrome susceptibility. Methods of ischemic stroke, 84 patients with blood stasis syndrome ( case group), ischemic stroke patients with 143 cases of non-blood stasis syndrome (control group), drawn peripheral venous blood extracted DNA, using polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) detection of MTHFR gene C677T polymorphism type. Results Group 2 distribution of MTHFR C677T genotype was significantly (χ2 = 12.618, df = 2, P = 0.002), mutant allele frequencies in case group than control group (respectively, 39.29% and 24.48%), TT genotype and occurrence of ischemic stroke was significantly related to blood stasis syndrome (OR = 3.730,95% CI was 1.229 ~ 11.318, P = 0.014). Conclusion TT MTHFR gene may be the type of ischemic stroke stasis predisposing risk factor for disease. [Keywords:] ischemic stroke, blood stasis, methylenetetrahydrofolate reductase gene, single nucleotide polymorphism Abstract: Objective To explore the relationship between the C677T single nucleotide polymorphism of N5, N10-methylenetetrahydrofolate reductase (MTHFR) and blood stasis syndrome (BSS) of ischemic stroke (IS). Methods The MTHFR C677T genotypes in 84 patients of IS of BSS type (BSS-IS group), 143 patients of IS of non-BSS type (non-BSS-IS group) were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. Results The constituent ratio of MTHFR CC, CT and TT genotype was significantly different between two groups (χ2 = 12.618, df = 2, P = 0.002). The frequencies of T allele in patients with BSS-IS (39.29%) were higher than that of non-BSS-IS group (24.