Klebsiella pneumoniae extended-spectrum β.docVIP

 PAGE \* MERGEFORMAT 13 Klebsiella pneumoniae extended-spectrum β Author: CHENG Xun-min, Zhang Qi, Xu Yuan-hong, Li Min, Zhu Suzhen, Shen Ji-recorded [Keywords:], Klebsiella pneumoniae Detection of extendedspectrum betalactamases and analysis of antibiotic resistance in clinical isolates of Klebsiella pneumoniae [Abstract] AIM: To study the phenotype and genotype distribution of extendedspectrum betalactamases (ESBLs) producing Klebsiella pneumoniae (K. pneumoniae) and its drug resistance. METHODS: Seventyseven strains of K. pneumoniae were determined by 1999 NCCLs methods and the genotype of ESBLs was amplified by polymerase chain reaction (PCR). The antibiotic susceptibility of bacteria was determined by microdilution. RESULTS: In the 77 positive K. pneumoniae isolates of the initial screen test, 45 strains (58.1%) were confirmed by cefotaxime and cefotaximeclavulanic acid but only 27 strains were confirmed by ceftazidime and ceftazidimeclavulanic acid. PCR showed that TEM, SHV, CTXM or OXAtype ESBLs in the clinical strains were 19, 8, 36, 11 strains respectively. CONCLUSION: K. pneumoniae is one of the most common ESBLsproducing strains . The rates of ESBLsproducing K. pneumoniae significantly increase in recent years and CTXM is most common genotype. Some strains carry two or more genotypes. Imipenem is the most susceptible antibiotic. [Keywords] Klebsiella pneumoniae; betalactamases; phenotype; genotype; drug resistance; antibiotic [Abstract] Objective: To understand the producing extended-spectrum β -lactamase (ESBLs) producing Klebsiella pneumoniae phenotype and gene distribution and resistance to commonly used antibiotics. Methods: The 1999 NCCLS recommended screening test from 77 strains of Klebsiella pneumoniae producing ESBLs were screened out of suspicious bacteria, and used confirmatory test to be confirmed; with the PCR method of amplification of their genotypes; to do with the micro-dilution susceptibility test. Results: The screening strai