Mass spectrometry analysis of proteins.doc

 PAGE \* MERGEFORMAT 11 Mass spectrometry analysis of proteins The highest content of protein is the organism, the most important biological macromolecules, present in all living cells, about the quality of dry cell weight of 50% or more. With the life sciences and the rapid development of biotechnology, bio-organic mass spectrometry has become Mass of the most active and most dynamic areas of advanced research. This paper briefly reviews the peptide and protein mass spectrometry analysis of the characteristics of biological macromolecules, methods, and the principle of protein mass spectrometry, methods and applications, and prospects of its development prospects . 1 Characteristics and methods of mass spectrometry 1.1 MS analysis with high sensitivity, sub-microgram level for the sample to provide information that can most effectively and Chromatography for complex system or structure identification of trace substances in the determination of both accuracy, ease , fast and good universality. 1.2 MS analysis method of soft ionization mass spectrometry techniques are the following: (1 electrospray ionization mass spectrometry, (2 matrix-assisted laser desorption ionization mass spectrometry, (3 fast atom bombardment mass spectrometry, (4-ion spray ionization mass spectrometry, ( 5 atmospheric pressure ionization mass spectrometry. In the past three most recent studies, the application most widely used. 2 protein mass spectrometry 2.1 The principle of the principle of protein by mass spectrometry ionization sources of protein molecules into the gas phase ions, then use the mass spectrometer’s field, the magnetic field will have a specific mass and charge ratio (M / Z value of the protein ions separately, after the ion detector separation of ions collected to determine the ion M / Z value of identification of unknown proteins. 2.2 protein and peptide sequence analysis of the existing peptide and protein sequencing methods