Matrine on the proliferation of human ACHN renal cancer cell growth inhibition and its mechanism.doc

 PAGE \* MERGEFORMAT 17 Matrine on the proliferation of human ACHN renal cancer cell growth inhibition and its mechanism Of: types of iron, pay the German, the Niu Qiang, Li and Cheng, Peng, sweet people, Zi-Ming Wang [Abstract] Objective To observe the people of matrine on in vitro growth inhibition of ACHN renal cancer cells and to explore its possible mechanism. Methods were stimulated with different concentrations of matrine in human renal carcinoma ACHN cell line 24,48 , 72,96 h, matrine observed by MTT cytotoxicity against ACHN; transmission electron microscopy and flow cytometry of matrine on apoptosis of ACHN cells; immunocytochemistry to detect Bcl matrine on ACHN cells -2, Bax protein expression. The results of different concentrations of matrine on the ACHN cell proliferation were certain, and a dose and time dependent; matrine significantly induced apoptosis of ACHN cells; flavescens After the base effect, ACHN cells were significantly increased protein expression of Bax, Bcl-2 protein expression in a downward trend, but no significant difference. Conclusions of matrine on the proliferation of human renal carcinoma ACHN cells significantly inhibited the proliferation of its mechanisms may regulate the ratio of Bcl-2/Bax protein expression and induce apoptosis. [Keywords:] matrine; renal cell carcinoma; ACHN cell lines ABSTRACT: ObjectiveTo observe matrine s inhibitory effect on the growth of ACHN human renal cell carcinoma in vitro and explore its possible mechanism.MethodsThe cell line of ACHN human renal cell carcinoma was treated with matrine of different concentrations for 24, 48, 72 and 96h , respectively. MTT assay was used to evaluate the cytotoxic effects of matrine on ACHN cells. The ACHN cell apoptosis induced by matrine was detected with transmission electron microscopy and flow cytometry. The expressions of Bcl-2 and Bax proteins were evaluated with immunocytochemical technique. ResultsMatrine of different concentrations had