Matrine oxymatrine sophoridine on macrophage activity and tumor necrosis factor-α in_0.doc

 PAGE \* MERGEFORMAT 13 Matrine oxymatrine sophoridine on macrophage activity and tumor necrosis factor-α in Of: Liu Taihua, Liu Defang, WANG Jun, Wang Xiaojun, Luo Chen [Abstract] Objective To observe the matrine, oxymatrine and Sophoridine RAW264.7 macrophage activity and tumor necrosis factor - @ (TNF-@). Methods 6 well plate cultured macrophages RAW264 .7, different concentrations of matrine, oxymatrine and Sophoridine and RAW264.7 macrophages were cultured 1 d, MTT assay using different concentrations of matrine, oxymatrine and Sophoridine RAW264.7 macrophage activity, collection of culture medium by ELISA, each culture medium content of TNF-@. Results of different concentrations of matrine, oxymatrine and Sophoridine macrophage RAW264 .7 different degrees of inhibition activity, different concentrations of matrine, oxymatrine and Sophoridine RAW264.7 macrophages inhibit the secretion of TNF-@, the greater the concentration, the stronger the inhibition, bitter reference base RAW264.7 macrophages inhibited the secretion of TNF-@ is more obvious. Conclusion matrine, matrine and sophoridine oxide can inhibit the activity of RAW264.7 macrophages and the secretion of TNF-@, bitter inhibition is more obvious reference base. [Keywords:] matrine, oxymatrine, sophoridine, macrophages, tumor necrosis factor - @, psoriasis Abstract: Objective To observe the effects of matrine, oxymatrine and sophordine on activity and TNF-@ secretion of macrophage RAW264.7. Methods Macrophage were cultivated in 6 holes board and cultivated 1 day with different concentration matrine, oxymatrine and sophordine. Macrophage activity was measured with MTT and content of TNF-@ in collected culture medium was measured with ELISA. Results Macrophage activity and the function of secreting TNF-@ was restrained by matrine, oxymatrine and sophordine. The higher concentration of matrine, oxymatrine and sophordine was, the more intense the inhibiting effect was. The effect of matrine