Matrix-assisted laser desorption ionization time of flight mass spectrometry analysis of notoginseng and Aplysia neural connective components of the saponins.doc

 PAGE \* MERGEFORMAT 23 Matrix-assisted laser desorption ionization time of flight mass spectrometry analysis of notoginseng and Aplysia neural connective components of the saponins Of: Feng Li Jian Huang Lin Zhuohui Qin Qing Huang [Abstract] matrix-assisted laser desorption ionization time of flight (MALDI TOF mass spectrometry desorption thirty-seven efficiently extract (Panaxnotoginseng Extraction, PNE mixture of saponins in the saponin molecule ion, and mass analyzer for detection and analysis. Selection of MALDI TOF mass spectrometry direct analysis of chromatographic purity of pure saponins, the detection sensitivity is better than high performance liquid chromatography (RP HPLC. optimize the extraction of traditional Chinese medicine thirty-seven (Panax notoginseng, PN) of the mixture of saponins, the choice of MALDI TOF mass spectrometry analysis of PNE in direct the type and relative content of saponins found PNE contains at least 20 different molecular structure of the saponin fraction of ginseng saponins (Ginsenoside) Rg1 and PNS (NotoginsenosideR1 were relatively high. use thin chromatography (TLC R1 in the preparation of PNE saponin. MALDI TOF mass spectrometry of locus coeruleus back anal Aplysia (Notarcus leachiicirrosus Stimpson, NLCS) neural connective in the minim R1, composition and distribution. establish PNE saponin fingerprints, and suitable for evaluation of traditional Chinese medicine Panax quality and Analysis of the amount of saponins in vivo metabolism of micro processes and mechanisms. [Keywords:] notoginseng saponins, matrix-assisted laser desorption ionization time of flight mass spectrometry, fingerprint 1 Introduction Ginseng (Ginsenoside) and notoginseng (Panax notoginseng) is one of valuable Chinese herbs commonly used, mainly for the treatment of various blood stasis of the disease and traumatic injury, stasis, swelling, pain, bleeding with blood stasis, and strengthening the immune system without leaving and