Matrix metalloproteinase-9 in asthmatic rat polymorphonuclear cells.doc

 PAGE \* MERGEFORMAT 14 Matrix metalloproteinase-9 in asthmatic rat polymorphonuclear cells Of: gold micro-Ying, Ye Hui, Tong Xiasheng, Wangen Zhi, Guo Haiyuan, Ruanzheng Ying Jin Xiaohong [Abstract] Objective: To observe the matrix metalloproteinase 9 (MMP-9) in the asthmatic rat blood neutrophils (PMN) in the expression of PMN can participate through the synthesis of MMP-9 in the pathogenesis of asthma. Methods: Healthy male SD 30 rats were randomly divided into the asthma group (A group, budesonide treatment group (B group and control group (C group, with ovalbumin (OVA sensitized and challenged the method to establish the asthma model. separation and purification of blood PMN, immune cells chemical detection level of MMP-9 expression, ELISA assay of serum matrix metalloproteinase inhibitor -1 (TIMP-1 concentrations. Results: A group (0.196 + -0.011, OD value of PMN MMP-9 expression was significantly higher than C group (0.100 + -0.010, OD values #8203;#8203;(P lt;0.01, B group (0.135 + -0.008, OD value of the PMN MMP-9 expression was significantly lower than the A group but higher than C group (all P lt;0.01.A group Serum TIMP-1 was (34.96 + -4.54) ng / mL, the expression level was significantly higher than the C group (26.14 + -3.43) ng / mL (P lt;0.01, B serum levels of TIMP-1 expression was significantly lower than A group but higher than C group (P lt;0.05 or P lt;0.01.MMP-9 and TIMP-1 expression was significantly correlated (n = 29, r = 0.713, P lt;0.01). Conclusion: PMN synthesis of asthmatic rats function of increased MMP-9, PMN may be partly by increasing the synthesis of MMP-9 in the pathogenesis of asthma, this function can be partially inhibited by budesonide. [Keywords:] asthma, MMP-9, TIMP-1, PMN, rat Abstract: Objective: To observe the expressions of matrix metalloproteinase-9 (MMP-9) in blood polymorphonuclear leukocyte (PMN) of asthmatic rats. And to approach whether PMN could participate in asthma pathogenesis through synthesizi