Melatonin in vitro directed differentiation of bone marrow mesenchymal stem cells.docVIP

 PAGE \* MERGEFORMAT 10 Melatonin in vitro directed differentiation of bone marrow mesenchymal stem cells OF: He Wei, Shen Tiecheng, Di Rong Branch, Yang Yong, Zhang Zhijian [Abstract] Objective: To investigate melatonin (melatonin, MT in vitro rat bone marrow mesenchymal stem cells (marrow mesenchymal stem cells, MSCs) differentiation into neural cells at different times of the situation. Methods: The isolated cell culture and purification of large Rat MSCs, as the inducing agent to induce MT MSCs to differentiate into neural cells. 3,7,10,14 d after induction, respectively, by immunofluorescence determination of neural stem cell specific markers (nestin), neuron specific marker ( neurofilament, NF), and specific marker of astrocytes (GFAP) expression. Results: MT induction and differentiation 3 d, part of the triangular cell body contraction, and some become a simple bipolar or multipolar cells. induced cells began 3 d expression of nestin, 7 ~ 14 d expressed nestin and NF, cells do not express GFAP. CONCLUSION: MT can induce MSCs to differentiate into neural cells. [Keywords:] bone marrow mesenchymal stem cells, melatonin, neural cell [Abstract] Objective: To explore MT introduce marrow mesenchymal stem cells into neurons on different time. Methods: By using cell culture method, rat MSCs were isolated and purified, The rat MSCs were induced with MT.The expression of nestin, NF and GFAP in the differentiated cells was detected by immunofluorescence after introduced 3,7,10,14 days. Results: Three days after induction with MT some neurons were found to contract, refract more light and protrude and bulge.Some neurons contracted to atriangular shape, and some others became simple bipolar or multipolar cells. After being induced for 3 days, the cells expressed nestin.After 7 ~ 14 days, the cells expressed nestin and NF, but didn’t express GFAP. Conclusion: MT can introduce MSCs into neurons. [Keywords:] bone marrow mesenchymal stem cells; melatonin;