Microwave antigen retrieval with high temperature and pressure method of.doc

 PAGE \* MERGEFORMAT 8 Microwave antigen retrieval with high temperature and pressure method of [Abstract] Objective: To investigate the method and microwave antigen retrieval method of high temperature and pressure effect. Methods: Application of microwave radiation method, the direct boiling pressure cooker antigen retrieval method, line by immunohistochemistry and compared the effects of their application. Results: Microwave repair backgrounds were relatively clear, positive cells in the biopsy specimen should appear in the expression can be seen, but not obvious. The use of pressure cooker repair, so clean and repair the effect of the background clear and strong positive signal. Conclusion: The high-temperature high-pressure cleaner than the microwave method Slice the background, color contrast and clear structure, positioning accuracy, ease of observation, methods, and stable, reliable, and can improve the accuracy of pathological diagnosis. [Keywords:] antigen retrieval immunohistochemistry Immunohistochemical staining technique for its simple operation, high sensitivity and specificity, can shape and combine the advantages of antigen-targeted tumor is widely used in clinical and pathological diagnosis and differential diagnosis [1]. Therefore, immunohistochemical staining reliability of the results has been greatly concerned that the current operation of the most prominent issue is the question of antigen retrieval, we compared the commonly used antibodies pressure cooker, microwave heating and high temperature and pressure two kinds of repair methods, first results summarized below. 1 Materials and methods 1.1 Instrument Commercially available household pressure cooker, working pressure of 90 kPa, Whirlpool microwave oven. 1.2 Reagents ER, PR, C-erbB-2 reagents for the Beijing Golden Bridge Biotechnology Co., Ltd. in fir production of reagents, heating repair solution for the citrate buffer (pH 6.0). 1.3 Materials Hospital to collect specimens