MTT colorimetric assay Honeycomb protein on leukemia cell proliferation △.doc

 PAGE \* MERGEFORMAT 4 MTT colorimetric assay Honeycomb protein on leukemia cell proliferation △ Author: Sherry Zhang Zhang Sheng-Ming Wang Qiang Nai-Fa Miao Tokihiko [Abstract] Objective: To study the Honeycomb protein on the proliferation of leukemic cells for the treatment of leukemia, new drugs. Methods: Red leukemia mouse model, using MTT assay Honeycomb protein on leukemia cell proliferation. Results: Honeycomb protein in low concentration group and the saline group showed no significant difference in the concentration of treated group compared with the saline group on the inhibitory effect of leukemia cells between the two groups was statistically significant, and chemotherapy drugs (DNR hormone) is still lagging behind in comparison. Conclusion: The Honeycomb protein on leukemia cells inhibit proliferation effect. [Keywords:] MTT colorimetric assay Honeycomb protein in the proliferation of Membership, according to medical records, Honeycomb with Qufeng, attacking poison, pesticides and other effects. Its composition is more complex, more current research is propolis, the study of their proteins reported rarely. In this study, was observed by using MTT Honeycomb protein on erythroleukemia cell proliferation. 1 Materials and methods 1.1 Materials 1.1.1 MTT: purchased in the United States Sigma, Inc.; triple lysate: SDS-butanol hydrochloride; RPMI1640: U.S. Gibco products; newborn calf serum: Hangzhou Jiangbin Bio-Technology Co., Ltd.; dimethyl sulfoxide (DMSO): United States Sigma products. 1.1.2 Instruments microplate reader; carbon dioxide incubator; SW-CJ-2FD-type double-sided clean bench: Suzhou. 1.1.3 EL9611 cell lines: Tianjin Institute of Hematology; Honeycomb protein (Nidus vespae Protein, NVP): self-made. 1.1.4 Animal Health of the BALB / c mice were 30, 6 ~ 8 weeks old, weight 18 ~ 22g, male and female open. 1.2 Experimental Methods 1.2.1 Preparation of erythroleukemia mouse model method [1]: ① erythroleukemia cell line will be w