Mulberry flavonoids and mechanism of anti-inflammatory activity in vitro.docVIP

 PAGE \* MERGEFORMAT 18 Mulberry flavonoids and mechanism of anti-inflammatory activity in vitro Of: Zhang Dandan Lingshuang, Zhang Hongping, Shi Haixia, Xueyong Liang, Yang Xiaolu, Xujin Wen, Bian card [Abstract] Objective To study in vitro of mulberry flavonoids and its mechanism of anti-inflammatory activity. Methods synergistic IFN-γ and LPS inflammation model in mice RAW264.7 cells; Griess Reaction of NO production in supernatants; FRAP determine cell Total antioxidant capacity; RT-PCR detection of iNOS, COX-2, HO-1, IL-1β, IL-6, TNF-@ mRNA expression; Western blot detection of iNOS, p-ERK protein expression. Results mulberry flavonoids dose-dependent inhibition of NO content in the supernatant, and no cytotoxicity, raise the total cellular antioxidant capacity, reduced iNOS, COX-2, IL-1β, IL-6 mRNA and iNOS, p-ERK protein expression, increased HO-1mRNA expression, but expression of TNF-@ mRNA had little effect. Conclusion mulberry flavonoids in part through the ERK MAPK signal transduction pathway inhibition of iNOS gene and protein expression to inhibit NO production, increased total antioxidant capacity of cells while down COX-2, IL-1β, IL-6 and other inflammatory mediators and upregulation of anti-inflammatory HO-1 expression in the media exert anti-inflammatory effect. [Keywords:] Inflammation; inducible nitric oxide synthase; cell factor; mulberry flavonoids; mechanism Abstract: ObjectiveTo characterize anti-inflammative effect of total flavones from Morus alba L (TFM) on RAW 264.7 macrophages stimulated with interferon-γ (IFN-γ) / lipopolysaccharide (LPS). MethodsUsing IFN-γ plus LPS to stimulate RAW 264.7 macrophages as inflammatory cell model. Griess reaction was used for nitric oxide measurement, MTT assay were used for cell proliferation and viability, RT-PCR was used for assaying mRNA expression and Western blot was used for protein expression. ResultsTFM dose-dependently inhibited both NO production and iNOS expression in IFN-γ + L