Mycobacterium tuberculosis to stimulate the recovery factor of human blood in vitro test of lymphocyte proliferation.doc

 PAGE \* MERGEFORMAT 7 Mycobacterium tuberculosis to stimulate the recovery factor of human blood in vitro test of lymphocyte proliferation [Abstract] Objective To evaluate the recovery factor of Mycobacterium tuberculosis immunity. Methods 65 cases of TB blood lymphocytes 1640, 3 days to stimulate the recovery factor protein in control without the recovery factor protein, MTT analysis the amount of lymphocyte proliferation. Results recovery factor The blood of TB patients to stimulate lymphocyte proliferation in varying degrees, compared with the control group there was significant difference (P lt;0.01). Conclusion The recovery factor has strong immunogenicity of protein [Keywords:] Mycobacterium tuberculosis; recovery factor; immunity [Abstract] Objective To evaluate the immunity of the resuscitation promoting factor of mycobacterium tuberculosis.Methods 65 blood lymphocytes of tuberculosis was cultured with 1640, we stimulated the three days with the resuscitation promoting factor, control without the resuscitation promoting factor protein, and MTT analysis of the amount of lymphocyte proliferation.Results The stimulation of blood lymphocytes of TB patients with resuscitation promoting factor had varied degrees of proliferation as compared with the control group significantly (P lt;0.01). Conclusion The resuscitation promoting factor protein has a strong immunogenicity. [Keywords:] mycobactreium tuberculosis; resuscitation promoting factor; immunocompetence Recovery factor protein of Mycobacterium tuberculosis is by far the only way for the recovery of dormant Mycobacterium tuberculosis protein, studies have shown [1], the recovery factor of Mycobacterium tuberculosis proteins (Rpf) subunit vaccine trial in mice a high immune response, in addition to Rpf C, but could induce antibodies IgG1 and IgG2 @, -IFN, IL-10, IL-12, to promote T cell proliferation. Therefore, we applied tuberculosis Rpf stimulated lymphocyte proliferation in blood lymphoc