Murine allogeneic bone marrow transplantation animal model aGVHD.doc

 PAGE \* MERGEFORMAT 17 Murine allogeneic bone marrow transplantation animal model aGVHD [Abstract] Objective: To establish a stable and reliable allogeneic bone marrow transplantation (allogeneic bone marrow transplantation, Allo-BMT) acute graft versus host disease (acute graft-versus-host disease, aGVHD) animal model for Allo-BMT in aGVHD ideal experimental platform for research. Methods: Male C57BL / 6 (H-2b) for the donor mice, female BALB / C (H-2d) as the recipient mice by mouse lethal total body irradiation (total body irradiation, TBI) ( 8.0 Gy) after pretreatment in the mix of different graft ratio of donor bone marrow cells and spleen cells to cause varying degrees of aGVHD. based on survival, WBC count, and other clinical and pathological examination to determine the extent aGVHD. Results: allogeneic bone marrow alone group only some of the mice appeared aGVHD, 75% of mice long-term survival (gt; 30 d). mixing different ratios of donor bone marrow cells and spleen cells in Allo-BMT mice and extent of the time there were aGVHD differences, of which the bone marrow and spleen cells of mice 1.5:1 or 1:1, can be relatively concentrated period of time in (8 ~ 15 d) aGVHD observed typical clinical and pathological changes. Conclusion: Allo-BMT aGVHD Models need to include a certain percentage of spleen cells for bone marrow and spleen cells of 1.5:1 or 1:1 for the Allo-BMT, can be used as an ideal animal model of aGVHD. [Keywords:] mice; allogeneic bone marrow transplantation; acute graft-versus-host disease; animal model Abstract: Objective: To establish a mouse model of acute graft-versus-host disease (aGVHD) after allogeneic bone marrow transplantation (Allo-BMT) for providing experimental studies of aGVHD. Methods: Male C57BL / 6 (H-2b) mice were used as donors and female BALB / C (H-2d) mice as recipients.All BALB / C (H-2d) mice were randomly divided into 7 groups and received total body irradiation (TBI) 8.0Gy at 4 ~ 6 hours prior to trans