Nasopharyngeal carcinoma DNA polymerase β gene mutation and EBV infection.doc

 PAGE \* MERGEFORMAT 8 Nasopharyngeal carcinoma DNA polymerase β gene mutation and EBV infection Author: Zheng, Li Shan, ZHAO Guo-Qiang, DONG Zi-ming, SONG Bao-Yi [Keywords:] nasopharyngeal cancer DNA polymerase β gene mutation in human nasopharyngeal cancer and its relationship with EBV infection [Abstract] AIM: To study DNA polymerase β (polβ ) gene mutation in human nasopharyngeal cancer and its relationship with EBV. METHODS: Twentysix cases of nasopharyngeal carcinoma tissues and corresponding normal epithelial tissues were collected to study DNA polβ gene mutation with PTPCR, PCRSSCP and DNA sequence analyzing techniques. DNA was extracted and amplified with EBV primers and the results were observed and analyzed. RESULTS: polβ gene mutation was detected by SSCP in 8 of the 26 nasopharyngeal cancer tissues (with mutation rate 30.8%) and there was no mutation in juxtacancerous tissues. Many types of mutation were found by DNA sequencing. EBVDNA positive rate was 92.3%. The results of experiment indicated that the cases of polβ SSCP abnormal were accompanied with EBV infection. CONCLUSION: There is polβ alternation in human nasopharyngeal carcinoma, which leads to the sequence alternation in amino acid and spatial structure change. These mutations and EBV infection may play an important role in tumor genesis. [Keywords] DNA polymerase β ; nasopharyngeal neoplasms; gene mutation; EBV [Abstract] Objective: To study the DNA polymerase of nasopharyngeal β (DNA polymerase β , polβ ) variation and its association with whether the correlation between EBV infection. Methods: RTPCR method (RT-DNA polymerase chain reaction), PCRSSCP (polymerase chain single strand conformation polymorphism analysis), DNA sequence analysis of 26 patients with nasopharyngeal carcinoma and adjacent tissue specimens polβ gene mutation. with EBV-specific PCR-amplification detection of EB virus, the analysis of EBVPCR positive and polβ mutation and the correlation between naso