Nasosinusitis Granules Extraction Process.doc

 PAGE \* MERGEFORMAT 7 Nasosinusitis Granules Extraction Process Author: Lee Chun-Li Ruiping LIU Su-xia Wang Yongze [Abstract] Objective Optimization nasosinusitis Granule extraction process. Methods of baicalin as an index, using high performance liquid chromatography determination of baicalin content; using orthogonal L9 (34) preferred nasosinusitis Granule the best extraction process. The results of baicalin in 0.177 2 ~ 1.772 0 μ g range a good linear relationship (r = 0.999 4, n = 7), the average recovery was 99.3%, RSD = 2.26% (n = 6); the optimal extraction process as: 10 times the amount of water extracted herbs 3 times, 1 h / times, the concentration of alcohol precipitation 50%. Conclusion The determination method is accurate, reproducible and stability in the extraction process was optimized, reasonable and feasible. [Keywords:] nasosinusitis Granule baicalin orthogonal extraction RP-HPLC Nasosinusitis Granules Prescriptions an effective treatment for nasosinusitis by baicalin, Angelica, Xanthium so on. Baicalin baicalin is has an antibacterial effect of the active ingredient, is also nasosinusitis Granule the main active ingredient. In order to improve the yield of baicalin, we use the orthogonal experiment on the effects of baicalin extraction conditions such as extraction times, boiling time, add water, the concentration of alcohol precipitation were studied to explore suitable for industrial production, simple process, high yield the extraction process. Are reported below. An apparatus and materials 1.1 Instrument Shimadzu HPLC system SPD-10AVD, SPD-10A VP UV detector, manual injector, N2010 chromatography workstation; 1810-BC Quartz Automatic double pure water distillation device (Jiangsu Jintan Baita quartz Glass Instrument Factory); TD200 / Electronic Balance (Balance Instrument Co., Ltd. Tianjin); TG328B-type analytical balance (Shanghai Precision Instrument Factory Branch); KQ-250B ultrasonic washing machine (Shanghai Branson Ultrasoni