New iron immobilized affinity chromatography Preparation and Evaluation of Monolithic Columns.docVIP

 PAGE \* MERGEFORMAT 12 New iron immobilized affinity chromatography Preparation and Evaluation of Monolithic Columns Of: Expenditure Security System Wang Hui, Yu-Kui Zhang Lihua LIANG Zhen Shan is also the beginning of [Abstract] N acryloyl succinimide as functional monomer was prepared by high-activity groups with the overall material matrix. Seq three amino acid as the ligand, through the immobilized Fe3 +, the development of an immobilized Fe3 + affinity chromatography (Fe IMAC) Preparation of monolithic columns. The whole column phosphorylated peptide not only has good selectivity, and the enrichment of large capacity, high recovery and good reproducibility. In addition, the use of the monolithic column realized the milk protein hydrolysates phosphorylated peptides selectively enriched. In this study, the overall development of the Fe IMAC column may be useful for the phosphorylation of proteins. [Keywords:] immobilized metal ion affinity chromatography; phosphorylated peptide; selective enrichment; the whole column; iron 1 Introduction Protein phosphorylation in signal transduction, metabolic regulation, regulation of cell function in life activities such as playing an important role [1,2]. However, phosphorylated protein / peptide levels are usually low and vulnerable in the mass spectrometry analysis of a large number of non-phosphorylated protein / peptide interference, so the development of selective enrichment for the phosphorylated peptide in-depth development of new materials for the phosphorylation of proteins is essential [3]. Immobilized metal affinity chromatography (IMAC) is the most commonly used in the phosphorylation of enrichment technologies. Usually by Fe3 +, Ti4 +, Zr4 +, Ga3 + and other metal ions, and sub-Amino c acid (NTA) of the sequestration to achieve metal ion In silica, resin or other matrix material on the agarose immobilized, and by metal ions and phosphorylated peptides in the sequestration PO3-4, to achieve