New methylenetetrahydrofolate reductase single nucleotide polymorphisms used to detect susceptibility to hematological malignancies.doc

 PAGE \* MERGEFORMAT 9 New methylenetetrahydrofolate reductase single nucleotide polymorphisms used to detect susceptibility to hematological malignancies Author: Bao-An Chen Mei-Ju Ji Ni Jiang HOU Peng Zu-hong Gao Chong Ding-Hua, Yun-Yu Wang Jun Jian Cheng Gang Abstract In this study a new type of methylenetetrahydrofolate reductase (MTHFR) single nucleotide polymorphism (SNP) detection methods, and used to detect the genetic susceptibility to hematologic malignancies. CDNA chip, according to principles of production of a gene chip, using two-color fluorescent probe hybridization for detection of SNP loci, sequencing of the chip to verify the accuracy of test results, and thus the region from 157 cases of Jiangsu Province, China healthy controls and 127 cases of patients with hematologic malignancies (30 patients with multiple myeloma, 28 cases of non-Hodgkin’s lymphoma, 22 cases of acute lymphoblastic leukemia, 40 patients with acute myeloid leukemia, 7 cases of chronic myeloid leukemia) of the MTHFR gene C677T polymorphic loci were detected. The results showed that wild-type, heterozygous type and mutant showed the superposition of fluorescent green, yellow and red. The results coincide with the results of sequencing chips. 677C and 677T in the cases and the control group gene frequencies were 58.7%, 66.9%, 41.3% and 33.1%, the difference was significant (χ2 = 4.077, P = 0.043). 677TT genotype occurs MM significantly increased relative risk (OR = 4.21; 95% CI = 1.50-11.83; P = 0.006). Conclusion: The microarray method is accurate, high-throughput and low price, suitable for large-scale SNP surveys a sample; C677T polymorphism changes affect the incidence of the risk of hematologic malignancies. 677TT genotype is a susceptibility factor in MM. Keywords: single nucleotide polymorphism; gene chip; two-color fluorescence hybridization; methylenetetrahydrofolate reductase; hematologic malignancy A New Method for 5,10-Methylenete