Notoginsenoside R1 in rats in vivo metabolite analysis.doc

 PAGE \* MERGEFORMAT 11 Notoginsenoside R1 in rats in vivo metabolite analysis [Abstract] Objective notoginsenoside R1 metabolism in rats, described notoginsenoside R1 metabolites in rats. Methods notoginsenoside R1 microbial transformation product as reference, the use of LC-ESI-MS / MS method to detect the metabolites in rats. The results were detected in rats 8 metabolites were identified using the reference standard of their structures were: 20 (S) - PNS R2, 20 (R ) - PNS R2, 20 (S) - ginsenoside Rh1, 20 (R) - ginsenoside Rh1, ginsenoside Rh4, the original Panaxatriol, ginsenoside F1 and 3, 12 - diol-E-dammarane 20 (22), 24 - diene-6-O--D-xylose - (1 2)--D-glucoside. Conclusion notoginsenoside R1 metabolites in rats more, and Microbial transformation products with a strong similarity. [Keywords:] notoginsenoside R1 metabolites by high performance liquid chromatography - mass spectrometry Abstract: ObjectiveTo investigate the metabolites of notoginsenoside R1 in rats. MethodsThe microbial transformation metabolites of notoginsenoside R1 were used as standard references. LC-ESI-MS/MS analysis was used in the metabolism studies of notoginsenoside R1 in rats. ResultsThe structures of eight metabolites of notoginsenoside R1 in rat feces were identified by comparison with the reference standards separated from biotransformation. Their structures were identified as 20 (S)-notoginsenoside R2, 20 (R)-notoginsenoside R2, 20 (S)-ginsenoside Rh1, 20 (R )-ginsenoside Rh1, ginsenoside Rh4, protopanaxatriol, ginsenoside F1 and 3, 12-dihydroxydammar-E-20 (22) -24 - diene-6-O--D-xylopyranosyl-(1 2)--D- glucopyranoside.ConclusionNotoginsenoside R1 is extensively metabolized in rat and has the same metabolic pathway with microorganisms to some extent. Keywords:: Notoginsenoside R1; Metabolites; LC-MS/MS Panax Panax notoginseng (Burk) FH Chen is a traditional rare medicinal herbs, with bleeding, stasis, swelling, pain and other effects. Notoginseng dammarane