Normal Wistar rat skin type immune Sporotrichosis observed.doc

Normal Wistar rat skin type immune Sporotrichosis observed Of: Wong Jing Wai ball, increasing Ru Xue Li-yan Zhang Xiaohui Feng Peiying Limei Rong seats [Abstract] [Objective] To observe the normal Wistar rat skin type immune Sporotrichosis in the pathogenesis of the feasibility of the model and experimental conditions. [Methods] Wistar rats were divided into experimental group (60) and control group (6), Wistar rats in each experimental group selected the back skin of two inoculation site: the roots of the right back and tail; produce Sporothrix schenckii strains SUMS0382 the spore suspension, and the concentration of bacterial suspension adjusted to the number of spores 1 108 / mL; Wistar rats in the experimental group, select the location of each spore suspension was injected subcutaneously 0.1 mL, caused by experimental infection; corresponding parts of the control group were injected 8.5 g / L NaCl solution of 0.1 mL. 12 weeks after inoculation rats daily activity, body weight, number and diameter of lesions, and in 2 weeks, 4 weeks, 8 weeks skin lesions were taken and tissue pathological fungal culture. [Results] lesions 6 days after infection began to appear, the number and size of lesions were in the 2nd week after infection and peaked. 1 week after infection, 33% (20/60) occurred in rats at the injection site lesions, 2 weeks after infection, 90% (54 / 60) lesions in rats appear at the inoculation site, the tail is much larger than the number of root lesions of the number of right-back lesions .91% (49/54) showed nodular lesions in rats .19% (10/54) lesions in rats showing repeated the process. lesional tissue fungal culture and histopathology were consistent with cutaneous Sporotrichosis change. [Conclusion] normal Wistar rat skin type immune Sporotrichosis reflect the pathogenesis of human disease the natural course of disease, normal Wistar rat skin type immune Sporotrichosis model is feasible. [Keywords:] Sporotrichosis, skin type; Wistar rats;