Obesity in rats by inhibiting contraction of ventricular myocytes and Ltype calcium channel.docVIP

 PAGE \* MERGEFORMAT 12 Obesity in rats by inhibiting contraction of ventricular myocytes and Ltype calcium channel On: Sun Qiang, Hu Hao, Zhou Yun, in Xiao Jiang, Wei-Jin Zang, Chen Chen [Abstract] Objective To study obesity, inhibin (obestatin) on myocardial contraction, intracellular calcium transients and membrane L-type calcium channels. Methods Single-cell contraction / fluorescence detection system and the edge of the whole cell patch clamp technique was obestatin on acute enzymatically isolated rat ventricular muscle cell contraction and calcium signals. the results of 10-7mol / L obestatin increased contraction in rat ventricular myocytes [(26.43 + -6.13)%] and intracellular calcium transient amplitude [(21.33 + -4.73%)], and increase the peak ICa L. Conclusion Obestatin open cardiac L-type calcium channels on the cell membrane, promoting calcium influx, enhanced ICa L in rat ventricular myocytes and intracellular calcium transients, thus enhancing contraction of myocardial cells . [Keywords:] obestatin; ventricular myocytes; calcium transient; L-type calcium current ABSTRACT: Objective To study the effects of obestatin on the contraction, calcium transient and L type Ca2 + current (ICa L) in rat ventricular myocytes. Methods The extent of contraction and Ca2 + transient changes were recorded with the motion edge detection system, and L type Ca2 + current was recorded with the whole cell patch clamp technique. Results Obestatin of 10-7mol / L increased the contraction amplitude of ventricular myocytes (26.43 + -6.13)% and Ca2 + transient amplitude (21.33 + -4.73)%, and enhanced ICa L peak. Conclusion Obestatin can strengthen contractility through increasing ICa L and Ca2 + transient in rat ventricular myocytes. KEY WORDS: obestatin; ventricular myocyte; calcium transient; L type Ca2 + currentSupported by the National Natural Science Foundation of China (No No and the Sci tech Research and Development Project of Shaanx