Of baicalin on the activity of Candida albicans.docVIP

 PAGE \* MERGEFORMAT 3 Of baicalin on the activity of Candida albicans [Abstract] Objective To test the use of spectrophotometry baicalin role in Candida albicans, its succinate dehydrogenase (SDH), cytochrome C oxidase (CCO), Ca2-Mg2-ATPase activity changes in order to explore anti-baicalin the role of Candida albicans target site, in order to explore the active components of Chinese medicine provide the basis for the mechanism of anti-fungal. Methods The effect of baicalin in the corresponding time of Candida albicans to prepare bacterial suspensions, repeated freeze-thaw grinding, differential centrifugation of mitochondrial extracts of Candida albicans; Lowry assay mitochondrial protein content; spectrophotometry SDH enzyme, CCO enzyme , Ca2-Mg2-ATPase activity. Results ① 0.25,0.5,1 mg / ml of baicalin concentrations of Candida albicans SDH activity was significantly lower than 0 mg / ml of baicalin concentration group (P amp;lt;0.05), and the higher the concentration of baicalin appeared SDH activity, the lower the trend of . Different time of Candida albicans SDH activity between the two groups no statistically significant difference (Pamp;gt; 0.05). ② Candida albicans by different concentrations of baicalin in different time, its CCO enzyme activity were no significant differences (Pamp;gt; 0.05). ③ 0.25,0.5,1 mg / ml concentration of baicalin group and 0 mg / ml concentration of baicalin group, Ca2-Mg2-ATPase activity significantly different, the higher the concentration of baicalin, Ca2-Mg2-ATPase activity the lower the role of the time on the Ca2-Mg2-ATPase activity did not significantly affect (Pamp;gt; 0.05). Conclusions ① baicalin has obvious effect of anti-Candida albicans; ② baicalin can reduce the SDH enzyme activity of Candida albicans; ③ baicalin on Candida albicans CCO was no significant effect on enzyme activity; ④ baicalin can reduce the Candida albicans Ca2 -- Mg2-ATPas [Keywords:] baicalin Candida albicans anti-fungal Abstract: