On the cytology combined screening of human papillomavirus cervical lesions detected.docVIP

 PAGE \* MERGEFORMAT 6 On the cytology combined screening of human papillomavirus cervical lesions detected [Keywords:] cytology screening for human papilloma virus cervical lesions October 6, 2008 published the first Nobel Prize winner in physiology and medicine, the German Harald. Zur. Wellhausen, to make people aware of the human papilloma virus (humanpapillomavirus, HPV) and cervical cancer, cervical cancer to become the only cause of all cancers present clear, only early prevention and treatment, only the complete eradication of cancer. In this study, the analysis in our hospital outpatient liquid-based cytology combined detection of human papilloma virus screening in cases of cervical lesions to explain the relationship between HPV and cervical lesions. Subjects and methods 1. Target: May 2005 ~ March 2009 in our hospital outpatient liquid-based cytology (liquid based cytologic test, LCT) abnormalities of the 1 284 patients, re-HPV testing. Aged 19 to 77 years ( 38.5 +10.3) years, 926 cases positive for HPV check point in the colposcopy biopsy of cervix. 2. Cytological and pathological diagnosis: routine disinfection and collection of cervical squamous neck tube column at the junction of exfoliated cells, sent to the laboratory diagnostic report made by TBS method [1]. 3. HPV genotyping: HPV DNA extraction (QIAGEN company QIAMINI kit), PCR amplification (U.S. MBI’s PE9600PCR amplification), nucleic acid hybridization fast back (Hong Kong Cape’s HPV genotyping microarray test kits and rapid nucleic acid hybridization HYBRIMAX medical instrument) line HPV genotyping. Results HPV subtypes by chip color distribution sites to determine the appropriate [2]. have 2 or more 2 or more for the type of infection. 4. Colposcopy and histological examination: LCT and positive for HPV colposcopy examination (Goldway Industrial Co., Ltd. of SLS = C 2000 e) Check, by 5% acetic acid test, iodine test to take part in the suspicious biopsy sent to pathol