Paeonol pairs of colorectal carcinoma HT29 cells bcl2 and bax gene expression in.docVIP

 PAGE \* MERGEFORMAT 4 Paeonol pairs of colorectal carcinoma HT29 cells bcl2 and bax gene expression in [Keywords:] Colorectal bcl  2 bax paeonol Resistance to chemotherapeutic drugs [1] and its toxicity has been the treatment of colorectal cancer are two problems, so in plants to find effective and side effects of anticancer drugs has become an important research topic at home and abroad. Studies have shown that paeonol (Paeonol, Pae) has a certain anti-tumor activity, intragastric administration has anti-tumor effect in mice liver [2]. We have reported that Pae could significantly inhibit the in vitro HT  29 colorectal cancer cell proliferation [3]. To further explore the possible mechanism of this inhibition, we conducted the following studies, the clinical application of Pae and provide theoretical and experimental basis. 1 Materials and methods 1.1 Cell Culture Conventional culture HT  29 cells, logarithmic growth phase cells used in the experiment. Cells were divided into experimental group and control group, the experimental group were added to different concentrations of Pae solution (respectively, 15.63mg / L, 62.5mg / L, 250mg / L), the control group by adding an equivalent medium. 1.2 Pae-induced apoptosis of 1.2.1 was observed under inverted microscope, conventional culture bottle culture HT  29 cells, inverted microscope, cell growth under continuous dynamic observation of the situation. 1.2.2 TUNEL assay of apoptosis cells were equipped with a cover slip of the six-well culture plates, adherent and divided into experimental group and control group, 48h after the coverslip removed, according to TUNEL kit instructions for processing. Light microscope observation, nuclei stained brown convicted of apoptotic cells were randomly selected five high-power microscope (* 200) vision, each vision count 200 cells, apoptosis index (AI) = number of apoptotic cells / Total cell count * 100%. 1.2.3 digestion of cell cycle detected by flow cyto