Pill from Yin Yang deficiency hypothalamus combination on dopamine and serotonin content.doc

 PAGE \* MERGEFORMAT 11 Pill from Yin Yang deficiency hypothalamus combination on dopamine and serotonin content Of: Wu Yong, Lee Wood, Zhuo Jia Lian, Chen Wei Xuan, Fan Mingwei [Abstract] Objective Shenqi Pill in its separate group deficiency in rats fed water extract were determined after administration of the dopamine in the hypothalamus of rats (DA, serotonin (5-HT content of kidney pills “from Yin Yang” mechanism of the treatment deficiency syndrome. Methods hydrocortisone 25 mg / (kg d injected rats, continuous 12 d, both parties split the group of kidney pill decoction orally, 21 days in the stomach, anesthetized rats, the hypothalamus removed, weighed, homogenized in 5% perchloric acid, high-speed supernatant after centrifugation at low temperature, high performance liquid chromatography DA ,5-HT’s content. Results Compared with normal rats, the hypothalamus in the DA ,5-HT levels increased by oral administration, each of its separate group of rats in the hypothalamus DA ,5-HT have different levels degree of reduction to the lowest levels of yin and yang dual complement group, while no significant decrease Yang group, which make up a group of yin and yang dual group 1 were lower than yang, yin and yang make up group 2 were lower than two-yang 2 groups was statistically significance. Conclusion reduced hypothalamic dopamine, serotonin levels, may be the Pill “from Yin Yang” combination on one of the mechanisms deficiency syndrome. [Keywords:] Qi Pill from Yin Yang deficiency syndrome serotonin dopamine high performance liquid chromatography Abstract: ObjectiveTo study the influence of compatibility of Shenqi Pill “Reinforcing Yang from Yin” on the contents of DA ,5-HT in hypothalamus of deficient kidney-yang Rats.MethodsThe rats were injected with cortisone at the dose of 25 mg / (kg d) for consecutive 12 days and adminstered with dismantled decoction at the same time. On the 21st day, the rats were sacrificed and the contents of DA ,5-HT i