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Pre-oxygen on cerebral cortical neurons injury.doc

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Pre-oxygen on cerebral cortical neurons injury

 PAGE \* MERGEFORMAT 15 Pre-oxygen on cerebral cortical neurons injury [Abstract] the effects of different concentrations of pre-oxygen, hypoxia and reoxygenation on cerebral cortical neurons injury. Methods: 72 adult male Wistar rats were randomly divided into A, B, C 3 groups, each group was divided into four subgroups. A Ⅰ ~ A Ⅳ (pre-oxygen) inhalation of sub-groups were 21%, 50%, 75% and 95% oxygen-30min; B Ⅰ ~ B Ⅳ (pre-oxygen  hypoxia) subgroup A group approach by inhalation of 5% oxygen and then 20min; C Ⅰ ~ C Ⅳ (pre-oxygen   hypoxia-reoxygenation) sub-group method of treatment according to group B, then 98% oxygen inhalation 20min. Cerebral cortex of rats in each group to take to measure lactic acid (LA), malondialdehyde (MDA), oxygen free radicals (OFR), total antioxidant capacity (T  AOC), total superoxide dismutase (T  SOD), catalase (CAT), glutathione peroxidase (GSH  Px) and amino acid content, or viability. By immunohistochemical staining of inducible nitric oxide synthase (iNOS). In the light microscope observation of morphological changes of neurons. Results: A Ⅰ , A Ⅱ sub-group of neurons no significant change, A Ⅲ , A Ⅳ sub-group of neurons in mild swelling. B group neurons swelling Evidently, B Ⅲ , B Ⅳ subgroup is more serious. C group neurons in the nucleus condensation, nucleolus reduced and disappeared, of which the lightest C Ⅱ subgroup, C Ⅲ , C Ⅳ sub-group of the most weight, that even the nuclear cracking, disappear, only the outline of the residual cell ghost cell. Compared with the A Ⅰ Asia, A Ⅲ ~ A Ⅳ subgroup OFR, iNOS, and LA increased (P amp;lt;0.05), prompted the three factors involved in the injury of brain damage; T  AOC increased (P amp;lt;0.05), may be damage factor is due to rise to the feedback of certain antioxidant factors of increase; T  SOD reduced (P amp;lt;0.05), may be related to an increase in the consumption. C Ⅰ ~ C Ⅲ subgroup of OFR and C Ⅰ , C Ⅳ subgroup of iNOS, as well as C Ⅱ

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