Pressure overload of Telmisartan on Myocardial expression of endothelin -1 and GATA DNA binding activity of.doc

 PAGE \* MERGEFORMAT 14 Pressure overload of Telmisartan on Myocardial expression of endothelin -1 and GATA DNA binding activity of Study: Law Chi Gou Xiao-Yan Li, Ping Zhou Yunzhen Hsi Yu Jiangheng [Abstract] Objective To study the pressure overload cardiac hypertrophy in rat heart tissue (GATA) DNA binding activity and endothelin (ET) -1 protein expression and significance of the changes observed telmisartan on blood pressure overload cardiac hypertrophy Hemodynamic parameters (systolic blood pressure (SBP), diastolic blood pressure (DBP) and mean pressure (MBP)), left ventricular mass index (LVMI), transverse diameter of myocardial cells (DC), myocardial GATA DNA binding activity and ET-1 protein expression. method was established by abdominal aortic banding pressure overload cardiac hypertrophy model. 24 healthy SD rats were randomly divided into three groups: sham operation group (n = 8); operation group (n = 8) ; telmisartan group (7 days after surgery to telmisartan 3 mg kg-1 d-1 orally 4 w, n = 8). Determination of rats SBP, DBP, MBP, LVMI and DC; Semi-quantitative immunohistochemical detection of cardiac tissue ET-1 protein expression, electrophoretic mobility shift analysis and detection of ET-1 and GATA binding activity of specific binding and GATADNA. Results Compared with sham surgery group SBP, DBP , MBP, LVMI, and DC were significantly increased (P all lt;0.05); light microscope, real heart, interstitial structural changes; myocardial expression of ET-1 and GATA DNA binding activity increased significantly (P all lt;0.05) ; myocardial ET-1 expression and LVMI and DC showed a significant positive correlation (r = 0.895, r = 0.899, P all lt;0.01); compared with the surgical group, telmisartan SBP, MBP, LVMI, DC , myocardial ET-1 protein expression and GATA DNA binding activities were significantly lower (P all lt;0.05). Conclusions (1) GATA DNA binding activities increased to increase the expression of ET-1 in rats with pressure overload