Pressure overload on myocardial high-energy phosphate metabolism.doc

 PAGE \* MERGEFORMAT 14 Pressure overload on myocardial high-energy phosphate metabolism [Abstract] Objective To investigate cardiac pressure overload hypertrophy in rats and distribution of high-energy phosphate content changes. Methods Adult male SD rats with abdominal aorta constriction, after 5 weeks and 15 weeks of observation of hemodynamic parameters in rats , ventricular remodeling index, density gradient extraction of rat heart mitochondria, high performance liquid chromatography measurement of myocardial tissue and myocardial mitochondrial high-energy phosphate content. Results After 5 weeks after abdominal aortic constriction of left ventricular hypertrophy, postoperative 15 weeks, and determined to increase the dysfunction, CAA5 week group and the CAA15 week myocardial tissue ATP, ADP, AMP and total adenine nucleotide pool levels decreased, and the CAA15 weeks of myocardial PCr decreased, the total adenylate pool and ATP content is low in CAA5 week group, CAA15 week group mitochondria ATP, ADP, AMP and total adenine nucleotide pool decreased. Conclusion pressure overload may affect the myocardial high-energy phosphate content and distribution, energy consumption increased myocardial hypertrophy and energy production and reserves are insufficient, may be the cardiac hypertrophy and cardiac dysfunction occurred one of the mechanisms. [Key Words] high-energy phosphate ventricular hypertrophy in rat mitochondria Abstract: Objective To explore the effects of pressure overload on high energy phosphates content and its distribution of left ventricular hypertrophy in rats. Methods Male SD rats were randomized into 4 groups: coarctation of abdominal aorta 5week group (CAA5 group), 15weekgroup (CAA15 group) and sham operation group (SH5, SH15group). The hemodynamics and ventriclar remodeling parameters were measured. Ventricle of heart were removed and mitochondria were isolated by centrifugation. The size of adenine acid pool (ATP, ADP, AMP) and creati