Protein -1 sodium hydrogen exchanger inhibitor dimethyl amiloride on pHi changes HL-60-ADM cells and cell proliferation apoptosis.docVIP

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2017-05-05 发布于浙江
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Protein -1 sodium hydrogen exchanger inhibitor dimethyl amiloride on pHi changes HL-60-ADM cells and cell proliferation apoptosis.doc

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Protein -1 sodium hydrogen exchanger inhibitor dimethyl amiloride on pHi changes HL-60-ADM cells and cell proliferation apoptosis

Protein -1 sodium hydrogen exchanger inhibitor dimethyl amiloride on pHi changes HL-60/ADM cells and cell proliferation apoptosis Author: Chang Cheng, Pei-yan Kong, Xing-hua, PENG Xian-gui, Liu Lin, Liu, Zeng DF, Liang Xue, Wang Qingyu 【Abstract】 In order to explore the NHE protein -1 (NHE-1) inhibitor dimethyl amiloride (DMA) on the HL-60/ADM cell pHi changes and cell proliferation, apoptosis, in a sensitive HL - 60 cells as controls, with different concentrations of DMA intervention, fluorescent indicator (BCECF / AM) test to detect cell pHi, micro-thiazole blue method (MTT) test cell growth inhibition rate of cell cycle detected by flow cytometry , TUNEL assay in situ cell apoptosis, compared HL-60/ADM cells and HL-60 cells, the difference between. The results showed that: HL-60/ADM cells pHi compared with HL-60 cells were significantly increased; DMA under, HL-60/ADM cells pHi lower rate, growth inhibition rate, apoptotic rate was significantly higher than HL-60 cells; when the DMA concentration of 100-150 μmol / L, when, HL-60/ADM cells in G0/G1 phase cell ratio of the rate of increase, S phase and G2 / M phase cell percentage reduction ranges were higher than HL-60 cells. Conclusion: NHE-1 inhibitor DMA caused a pHi decrease HL-60/ADM can inhibit cell growth and induce apoptosis; and the DMA on the HL-60/ADM cell growth inhibition was significantly higher than HL-60 cells . Keywords: sodium / hydrogen exchanger protein -1 Effect of the NHE-1-Specific Inhibitor DMA on pHi, Proliferation and Apoptosis of HL-60/ADM Cells In Vitro Abstract The aim of this study was to evaluate the effect of dimethyl amiloride (DMA), a specific inhibitor of Na / H exchanger-1 (NHE-1), on intracellular pH value (pHi), proliferation and apoptosis of HL-60 / ADM cells in vitro. After treatment with DMA at different doses, pHi of HL-60 and HL-60/ADM cell lines were determined by using pH-sensitive fluorescence dye BECEF-AM; the rate of growth inhibiti