Purslane active ingredient in vivo anticancer effects of the initial screening.docVIP

 PAGE \* MERGEFORMAT 14 Purslane active ingredient in vivo anticancer effects of the initial screening Of: Yuping, Zengxian Wei, Ye Jun, Su Hu, Liu Hua, Zhou Chunli [Abstract] Objective To observe the active ingredient purslane on human lung adenocarcinoma cell line (A-549 cell), human laryngeal epidermoid carcinoma cell line (Hep-2 cell), human cervical cancer cell lines (Hela cell) and human malignant embryonic rhabdomyosarcoma cells (RD cell growth. Methods In vitro culture conditions with different concentrations of active ingredients purslane 4 cancer treatment, by brominated diphenyl dimethyl thiazolyl tetrazolium (MTT test cell method proliferation, while dealing with S180 tumor-bearing mice, mice concept Chahe weight, tumor weight, mortality and inhibition rate. Results purslane alkaloids on in vitro culture of the A-549 lung cancer cells, Hela cells and Hep -2 cells were obviously inhibited, Polysaccharide of Hela cells was strongly inhibited, purslane fatty acids on Hep-2 cells have a certain inhibitory effect on RD cells purslane flavonoids have a strong inhibition, and a dose dose effect, high concentrations of strong inhibition. Conclusion purslane active ingredient can kill cancer cells selectively, with the potential value of further research. [Keywords:] purslane, active ingredients, brominated diphenyl dimethyl thiazolyl tetrazolium test, anti-tumor activity Abstract: ObjectiveTo observe the effects of the active constituents from Portulaca oleracea L. on the proliferation of four human tumor cell lines. MethodsThe anti-proliferation activities of active constituents from Portulaca oleracea L. upon A549 cell, Hela cells, Hep-2 cell and RD cell were analyzed by MTT in vitro. Body weigt, tumor weigt, inhibition rate and death rate of these active constituents from Portulaca oleracea L. on the transplanted tumor cells of Sarcinoma 180 were observed in mice.ResultsRaw screening of the four compounds by MTT indicated polysaccharides, fl