Qi deficiency and blood vessel smooth muscle cell bcl2 and bax apoptosis-related factor expression.doc
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Qi deficiency and blood vessel smooth muscle cell bcl2 and bax apoptosis-related factor expression
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Qi deficiency and blood vessel smooth muscle cell bcl2 and bax apoptosis-related factor expression
[Abstract] Objective To observe with Qi apoptosis of vascular smooth muscle cells and bcl 2, bax-related apoptosis factor expression. Methods of hunger, fatigue, cold, shock and other comprehensive factors method to copy a rat model of Qi Deficiency to observe the endometrial morphology of rat thoracic aorta to detect the apoptosis index of vascular smooth muscle cells and bcl 2, bax protein expression. the result was Qi and Blood intimal proliferation of vascular smooth muscle cells increased expression of bcl 2 protein (96.15 + -0.02 vs 21.13 + -0.05); bax protein expression decreased (1.05 + -0.12 vs 68.06 + -0.06); bcl 2/bax higher (2.92 + -0.67 vs 0.01 + -0.01) (P lt;0.05). Conclusion The existence of qi deficiency blood stasis rats vascular smooth muscle cell proliferation and pathophysiological basis of severe disorders.
[Keywords:] with Qi; vascular smooth muscle cells; cell apoptosis
[Abstract] Objective To observe the apoptosis and the expression of of bcl 2 and bax in the vascular smooth muscle cells of rats with qi deficiency and blood stasis syndrome. Methods The rats model with qi deficiency and blood stasis syndrome were induced by exertion, starvation , cold and high fat food. The morphology of the thoracic aorta intima was observed, and the apoptosis index (AI) and the expression of protein bcl 2 and bax in the vascular smooth muscle cells were measured. Results The thoracic aorta intima apparently proliferated in model group (P lt;0.05), the level of protein bcl 2 in the vascular smooth muscle cells was significantly higher (96.15 + -0.02 vs 21.13 + -0.05, P lt;0.05) and the level of protein bax in the vascular smooth muscle cells was significantly lower than that of normal control group (1.05 + -0.12 vs 68.06 + -0.06, P lt;0.05). The ratio of bcl 2 and bax was higher (2.92 + -0.67 vs 0.01 + -0.01, P lt;0.05). Con
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