Qingyang parameter extraction process of Ultrasonic saponin.doc

 PAGE \* MERGEFORMAT 9 Qingyang parameter extraction process of Ultrasonic saponin Of: Zhoujin Kang, He Jun, Liang Zhiyuan, Ran Xiaoyan [Abstract] Objective To determine the ethanol solution as the solvent on the extraction of Qingyang parameters parameters sapogenin. Methods of ultrasonic power and ultrasonic treatment method, time, alcohol concentration, solid to liquid ratio, extraction times and to optimize the solution acidity and other factors. The results extracted parameter defined as: ultrasonic power 400 W, time 40 min, 50% ethanol concentration, solid to liquid ratio 1:10, the number of extraction 1, acidity 0.005 mol L-1HCl. Conclusions The method can be used for the Extraction of Qingyang parameters. [Keywords:] Qingyang Qingyang Participation Participation sapogenin Extraction Cynanchum otophyllum Scheid Qingyang Participation Division for the dill japonica Cynanchum species, meat has been named Bo Yi Qi medicine, Chinese medicine called Qingyang Participation, River parameters, mainly distributed in Yunnan, Guizhou and other places. The sweet and bitter of low back pain, uterine fibroids, tuberculosis, epilepsy embolism [1] have a therapeutic effect, so Qingyang ginseng extract pharmacological effects, chemical composition, etc. [2 5] of concern by the people. But Qingyang Participation Extraction Process rarely reported. We use ultrasound treatment method, ethanol as solvent to acid hydrolysis to obtain the stable transformation of saponins as an indicator, the use of high performance liquid chromatography (HPLC method was determined, and with reflux extraction and Soxhlet Comparison of extraction, and finally determine the parameters of ultrasonic treatment Qingyang extraction process conditions. 1 apparatus and materials 1.1 Instrument Angilent 1100 high performance liquid chromatograph (Agilent, USA), KQ-2250DB ultrasonic cleaning instrument (Kunshan Ultrasonic Instrument Co., Ltd.), electronic analytical balance (on Haiao