Qinling Mountain HCMV gN genotype distribution and its relationship with intrauterine infection.doc
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Qinling Mountain HCMV gN genotype distribution and its relationship with intrauterine infection
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Qinling Mountain HCMV gN genotype distribution and its relationship with intrauterine infection
Of: Xu Jing, Li Fen, Song Hui, Zhang Xinwen
[Abstract] Objective To explore maternal and child Shaanxi Qinling Mountains of human cytomegalovirus (HCMV) infection of epidemic strains of the envelope glycoprotein gN genotype distribution and outcome of intrauterine infection with HCMV-related. Methods 434 cases were detected by ELISA during pregnancy HCMV-IgM in serum of women and HCMV-IgG, determine the Qinling Mountains HCMV infection in pregnant women; detected by PCR in urine of pregnant women and newborns in the HCMV gN gene, using restriction endonuclease Sac , Sal and Sca on HCMV gN genes were restriction fragment length polymorphism (RFLP) analysis. Results 434 cases of pregnant women serum HCMV-IgM positive in 35 cases, positive rate of 8.06%; HCMV gN DNA positive in 25 cases, the positive rate was 5.76%. Freshmen children positive for HCMV gN DNA in urine in 17 cases, the positive rate was 3.92%. HCMV-IgM in 35 cases of positive pregnant women, newborn infants whose urine HCMV gN DNA matching positive in 11 cases, HCMV intrauterine transmission rate of 31.43%. 42 cases of HCMV gN DNA positive samples (25 pregnant women and 17 newborns) gN3 type 2 cases, gN4 40 cases, 8 cases of symptomatic HCMV infection in infants and 8 cases of asymptomatic infection in infants and their mothers have the same genotype gN4 type; 1 case of pathological jaundice and their mothers genotype gN3. symptoms and prognosis of neonatal infection with no correlation between genotypes. Conclusions Maternal and Child Shaanxi Qinling Mountains to gN4 type HCMV strains dominated. gN3 , gN4 genotype can lead to child transmission.
[Keywords:] human cytomegalovirus; congenital infection; glycoprotein N; genotyping
ABSTRACT: ObjectiveTo assess the distribution of human cytomegalovirus (HCMV) glycoprotein N (gN) genotypes in the maternal and infant HCM
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