Rabbit basilar artery after ligation of the scope of study of brain stem ischemia.doc

 PAGE \* MERGEFORMAT 7 Rabbit basilar artery after ligation of the scope of study of brain stem ischemia Author: ZHANG Cui-ying LU Yan-Zhen Fan Yimin Wang Limin Shang Gai-ping [Abstract] Objective: To observe the rabbit basilar artery after ligation of the brainstem ischemic range. Methods: In the beginning the Department of basilar artery occlusion produced ischemia animal model of brain stem, applied ink, and mecamylamine plastic perfusion blood vessels, light microscope analysis of changes in blood vessel density in the medulla oblongata in order to determine the scope and extent of ischemia, using Nissl staining of neurons morphological changes. Results: After ligation of the basilar artery, brain stem vascular density decrease (P amp;lt;0.05), the range of 0.2 mm ~ bolt after bolt before the 10 mm, including the medulla oblongata and upper pons; Nissl staining showed a number of ischemic neuronal cell body swelling, coloring shallow, Nissl body declined, and its gray value was significantly higher (P amp;lt;0.05). Conclusion: The initial ligation of the basilar artery can cause the Department of the upper medulla oblongata and pons of focal ischemic injury. [Keywords:] brainstem ischemia in rabbit basilar artery Abstract Objective: To observe ischemic coverage of brain stem after ligating basilar artery trunk in rabbits.Methods: Brainstem ischemic model were made by ligating the beginings of the basilar artery trunk, the blood vessels were perfused and showed with prepared Chinese ink and gelatin. The changes in the density of blood vessels were investigated for defining coverage and degree of ischemia. The morphology of neurons in brainstem were defined with Nissl stain.Results: The density of blood vessels notably decreased in brainstem from 10 mm rostral to the obex to 0.2 mm posterior to the obex including pons and part of superior medulla.The soma was swollen and the Nissl bodies decreased in some of neurons in the ischemic region of brainstem. Co