Rabbit model of assisted reproduction.doc

 PAGE \* MERGEFORMAT 13 Rabbit model of assisted reproduction Authors: LU Jing 1, namely, Yuan 1, Rong Ying 1, WANG Jie Xin 2, leaves United Shun [Abstract] Objective To understand the three kinds of culture medium on the effect of rabbit sperm in vitro and different superovulation superovulation drugs and female rabbits results and the relationship between changes in reproductive hormone levels. Methods of semen culture medium in three experiments. Pairs of female rabbits were injected PMSG 75u or HMG 20u 2 days, the first two days were injected HCG 80u superovulation experiment. Chemiluminescence was measured using rabbit reproductive hormone estrogen. The results of three kinds of medium 24h there was no difference in sperm motility, but the 48 and 72h there are differences in the BWW solution. Sperm motility in the 24,48, and 72h in BWW solution with the liquid and M199 in the F10 solution are different. PMSG injection of female rabbits the average number of 7.8 eggs, embryos number 6.6; of female rabbits injected HMG average number of 15 eggs, embryos number 25. HMG injection after PMSG or FSH, LH, P more significant increase, E2-6 also has increased, T no significant changes. Conclusion BWW medium is a good medium of sperm in vitro. The effect of HMG superovulation than PMSG. Intramuscular injection of HMG or PMSG on female rabbits FSH, LH, E2-6, P the average level of change. [Keywords:] rabbit; semen; gonadotropin; Superovulation; reproductive hormones [Abstract] Objective To observe rabbit sperm culture in three different culture media and find out the correlation between different superovulation drugs and the actual superovulation and the change of FSH, LH, E2-6, P and T in rabbit serum. Methods The semen was cultivated in three different culture media placed in a culture machine whose temperature was 37 ℃ . PMSG (75 U) or HMG (20 U) were intravascularly injected once a day for two days and HCG (80U) was injected on the third day.