RegCRD domain fragment of gene deletions and expression vectors obtained with the transfection.doc

 PAGE \* MERGEFORMAT 13 RegCRD domain fragment of gene deletions and expression vectors obtained with the transfection Of: Guo Ying, Xu Jiajia, business extension Fang, Li Nan, peak, Pei-Lin Huang [Abstract] Objective: To explore the gene splicing overlap extension PCR (SOE PCR gene deletion technology Building Reg CRD domain expression vector and transfected into low expression of Reg colorectal cancer cell lines. Methods: The SOE PCR method to obtain missing Reg CRD domain fragment of eukaryotic expression vector and transfected into low expression of Reg LoVo colorectal cancer cells, G418 selection, RT PCR detection and identification. Results: The screened by restriction endonuclease digestion of recombinant sequencing results consistent with the target sequence, recombinant vector was successfully constructed by pcDNA3.1 Reg domain transfected LoVo cells were positive for Reg domain expression. Conclusion: The use of SOE PCR technology can be successfully constructed gene deletion Reg CRD domain of expression vector. [Keywords:] Reg , carbohydrate recognition domain, colorectal cancer, gene transfer, gene splicing overlap extension PCR Reg gene family belonging to c lectin superfamily, involved in the liver, pancreas, stomach and intestinal cell proliferation and differentiation, and its effect may be related with the lectin domain of this structure [12], while the lectin has been considered as a tumor in vivo, in vitro screening, detection, diagnosis, prognosis and targeted treatment of biological markers and anti-tumor therapy to assess the risks and responses [3 5]. Reg gene family is the most studied Reg gene, which in tissue injury, diabetes, cancer and anti-apoptotic factors may play an important role, Reg Reg sequence homology with the structure similar to the most obvious feature is the region have c lectin [6]. In order to study gene Reg role in tumorigenesis, Domain Reg explore the function of genes, the study of gene sp