RegCRD domain fragment of gene deletions and expression vectors obtained with the transfection.docVIP

 PAGE \* MERGEFORMAT 13 RegCRD domain fragment of gene deletions and expression vectors obtained with the transfection Of: Guo Ying, Xu Jiajia, business extension Fang, Li Nan, peak, Pei-Lin Huang [Abstract] Objective: To explore the gene splicing overlap extension PCR (SOE PCR gene deletion technology Building Reg CRD domain expression vector and transfected into low expression of Reg colorectal cancer cell lines. Methods: The SOE PCR method to obtain missing Reg CRD domain fragment of eukaryotic expression vector and transfected into low expression of Reg LoVo colorectal cance