Ren Capsules Liver alcohol compound 5 the role of serum pharmacology.docVIP

 PAGE \* MERGEFORMAT 8 Ren Capsules Liver alcohol compound 5 the role of serum pharmacology Author: Zhang Bin, Dou Zhihua, Rowling, up-Kun Lin, especially bats instrument, CAO Wei-Ju [Abstract] Objective: To establish a five-jen alcohol compound capsule serum pharmacology research methods. Methods: CCl4-induced primary cultured rat liver cell injury model, rats were drug-containing serum for the body to liver injury in drug-containing serum on cell proliferation (MTT) and alanine aminotransferase (ALT) leakage of impact of two indicators, combined with drug-containing serum in the assay of schisandrin, respectively, a single-factor study, multi-factor orthogonal experiment, the preferred preparation method of drug-containing serum, and to determine the system to add the amount of serum. Results: The daily adult dose equivalent to 10 times the methanol extracts 8h intervals in two gavage administration to normal rats for 3 days, 2h after the last administration blood serum preparation containing more reasonable, the system by adding 10 % prepared under the condition containing serum could significantly increase the damage of liver cell proliferation, reducing cell ALT leakage. Conclusion: The preparation of the compound under conditions suitable for 5-jen alcohol capsule containing serum on liver cell injury has obvious protective effect, serum pharmacological research methods can be used to evaluate the efficacy of the preparation. [Keywords:] compound 5-jen alcohol capsule; hepatoprotective effect; containing serum; serum pharmacology [Abstract] Objective: To establish a serum pharmacology testing method for compound wurenchun capsules (CWC). Methods: Model of CCl4-induced injury of primary culture hepatocyte was established and rat was used as the donor to prepare serum containing CWC. With hepatocyte increment (MTT), alanine aminotransferase (ALT) in cell culture medium and the content of schisandrin in serum as indices, single-factor investigation and