RhD-positive blood cells in the preparation and application of shadow.docVIP

 PAGE \* MERGEFORMAT 11 RhD-positive blood cells in the preparation and application of shadow Authors: SHI Ming-Jun, Xiao Ying, Gui-hua Zhen, Zhang Guozhong [Abstract] Objective: To investigate the penetration with different concentrations of phosphate solution (PB) film prepared by RhD-positive blood cell antigen and its anti-D antibodies in the separation effect. Methods: Different concentrations of PB as a lysis buffer infiltration, genetic phenotype of CCDee of RhD-positive blood was prepared by O-type red blood cell ghost cell to observe its antigenicity; to affect blood cells solid-phase antigen, using affinity chromatography from the anti-D content of 0.814 mg / L in plasma of healthy individuals were isolated anti-D antibody detection of relevant indicators. Results: pH7.4, 30 mmol / L PB Preparation of blood film morphology integrity, and maintain a strong antigenicity; successful anti-D antibodies, the recovery rate is 84.65%, IgG monomer plus dimer content of 99.3% , does not contain anti-A, anti-B blood group antibodies, as well as IgA, IgM and hemoglobin. Conclusion: pH7.4, 30 mmol / L PB film preparation of blood cells can be used as solid phase antigen, and the price of plasma from the inefficient separation and purification of anti-D antibodies. [Keywords:] RhD antigen, anti-D antibodies affect blood cells, plasma [Abstract] Objective: To investigate the antigenicity of RhD ghost cells prepared with different concentrations of phosphate solution (PB) and its application in the separation of anti  D antibody from plasma with low antibody titer. Methods: Red blood cells of O group with positive RhD (genotype CCDee) were employed to prepare ghost cells using PB with concentrations of 40, 30 and 20 mmol / L respectively. Antigenicity of these cells was examined. Anti  D antibody was separated from the plasma containing 0.814 mg / L of anti  D by using affinity chromatography (AC) with the ghost cells as solid antigen. Results: 1. The ghost